W, X and Y are the intermediates in a biochemical pathway as shown below: S cdot

MCQ Practice

W, X and Y are the intermediates in a biochemical pathway as shown below: S cdot cdot cdot cdot cdot cdot cdot cdot to W to X to Y to Z Mutants auxotrophic for Z are found in four different complementation groups, namely Z1, Z2, Z3 and Z4. The growth of these mutants on media supplemented with W, X, Y or Z is shown below (Yes: growth observed: No: growth not observed): Mutants Media Supplemented with Mutants W X Y Z Z1 No No Yes Yes Z2 No Yes Yes Yes Z3 No No No Yes Z4 Yes Yes Yes Yes What is the order of the four complementation groups in terms of the step they block?

Subject: Biotechnology EngineeringTopic: Molecular Tools In Biotechnology

[S cdot cdot cdot cdot cdot cdot cdot cdot xrightarrow{{Z1}}Wxrightarrow{{Z2}}Xxrightarrow{{Z3}}Yxrightarrow{{Z4}}Z]

[S cdot cdot cdot cdot cdot cdot cdot cdot xrightarrow{{Z4}}Wxrightarrow{{Z2}}Xxrightarrow{{Z1}}Yxrightarrow{{Z3}}Z]

[S cdot cdot cdot cdot cdot cdot cdot cdot xrightarrow{{Z3}}Wxrightarrow{{Z1}}Xxrightarrow{{Z2}}Yxrightarrow{{Z4}}Z]

[S cdot cdot cdot cdot cdot cdot cdot cdot xrightarrow{{Z4}}Wxrightarrow{{Z1}}Xxrightarrow{{Z2}}Yxrightarrow{{Z3}}Z]

Correct Answer: B