The DNA sequence shown below is to be amplified by PCR: 5′ GCTAAGATCTGAATTTTCC . – OfficerPath

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The DNA sequence shown below is to be amplified by PCR: 5' GCTAAGATCTGAATTTTCC . . . . . . . . TTGGGCAATAATGTAGCGC 3' 3' CGATTCTAGACTTAAAAGG . . . . . . . . AACCCGTTATTACATCGCG 5' Which one of the following pair of primers can be used for this amplification?

Subject: Biotechnology EngineeringTopic: Molecular Tools In Biotechnology

A

5' GGAAATTCAGATCTTAGT 3' and 5' TGGGCAATAATGTAGCGC 3'

B

5' GCTAAGATCTGAATTTTC 3' and 5' GCGCTACATTATTGCCCA 3'

C

5' CGGAAATTCAGATCTTAG 3' and 5' GCGCTACATTATTGCCCA 3'

D

5' GCTAAGATCTGAATTTTC 3' and 5' TGGGCAATAATGTAGCGC 3'

Correct Answer: B

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