MCQ Practice

There are two different ways of determining the nucleotide sequence of a nucleic acid: the chemical quencing (Maxam-Gilbert) method and the enzymatic sequencing (Sanger) method. The basic principle/advantage of the Sanger method is

A

The differential interaction of the bases with particular dyes

B

Extension of a synthetic primer and reliable termination of DNA repair synthesis

C

The correlation of restriction sites with the end-label of the DNA

D

The ability to 'sequence' both strands of the DNA duplex simultaneously

Correct Answer: D