Which statement correctly describes sequential steps in cDNA cloning?

MCQ Practice

Subject: Biotechnology EngineeringTopic: Methods And Techniques In Applied Biology

Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector

mRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase, ligation to vector

mRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector

Double stranded cDNA synthesis, restriction enzyme digestion, addition of linkers, ligation to vector

Correct Answer: A

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