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Biotechnology Engineering

Molecular Tools In Biotechnology MCQs

Practice Molecular Tools In Biotechnology MCQs for competitive exams.

Molecular Tools In Biotechnology MCQs

Practice questions from this topic.

For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated DNA?

  1. A. KpnI
  2. B. DpnI
  3. C. XhoI
  4. D. MluI
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The DNA sequence shown below is to be amplified by PCR: 5' GCTAAGATCTGAATTTTCC . . . . . . . . TTGGGCAATAATGTAGCGC 3' 3' CGATTCTAGACTTAAAAGG . . . . . . . . AACCCGTTATTACATCGCG 5' Which one of the following pair of primers can be used for this amplification?

  1. A. 5' GGAAATTCAGATCTTAGT 3' and 5' TGGGCAATAATGTAGCGC 3'
  2. B. 5' GCTAAGATCTGAATTTTC 3' and 5' GCGCTACATTATTGCCCA 3'
  3. C. 5' CGGAAATTCAGATCTTAG 3' and 5' GCGCTACATTATTGCCCA 3'
  4. D. 5' GCTAAGATCTGAATTTTC 3' and 5' TGGGCAATAATGTAGCGC 3'
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Protein-DNA interactions in vivo can be studied by

  1. A. Gel shift assay
  2. B. Southern hybridization
  3. C. Chromatin immunoprecipitation assay
  4. D. Fluorescence in situ hybridization assay
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Restriction endonucleases from two different organisms that recognize the same DNA sequence for cleavage are called

  1. A. Isoschizomers
  2. B. Isozymes
  3. C. Concatemers
  4. D. Palindromes
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In order to identify the person who committed a crime, forensic experts will need to extract DNA from the tissue sample collected at the crime scene, and conduct one of the following procedures for DNA fingerprinting analysis

  1. A. Cut the DNA and hybridize with specific micro-satellite probes
  2. B. Cut the DNA and subclone the fragments
  3. C. Determine the sequence of the subclones
  4. D. B followed by C
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For amplification of GC rich sequences by polymerase chain reaction, identify the reagent that hinds and stabilizes AT sequences and destabilizes GC regions.

  1. A. Tetramethyl ammonium chloride
  2. B. Betaine
  3. C. 7-deaza-2'-deoxyguanosine triphosphate
  4. D. Sodium dodecyl sulphate
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W, X and Y are the intermediates in a biochemical pathway as shown below: $$S cdot cdot cdot cdot cdot cdot cdot cdot to W to X to Y to Z$$ Mutants auxotrophic for Z are found in four different complementation groups, namely Z1, Z2, Z3 and Z4. The growth of these mutants on media supplemented with W, X, Y or Z is shown below (Yes: growth observed: No: growth not observed): Mutants Media Supplemented with Mutants W X Y Z Z1 No No Yes Yes Z2 No Yes Yes Yes Z3 No No No Yes Z4 Yes Yes Yes Yes What is the order of the four complementation groups in terms of the step they block?

  1. A. [S cdot cdot cdot cdot cdot cdot cdot cdot xrightarrow{{Z1}}Wxrightarrow{{Z2}}Xxrightarrow{{Z3}}Yxrightarrow{{Z4}}Z]
  2. B. [S cdot cdot cdot cdot cdot cdot cdot cdot xrightarrow{{Z4}}Wxrightarrow{{Z2}}Xxrightarrow{{Z1}}Yxrightarrow{{Z3}}Z]
  3. C. [S cdot cdot cdot cdot cdot cdot cdot cdot xrightarrow{{Z3}}Wxrightarrow{{Z1}}Xxrightarrow{{Z2}}Yxrightarrow{{Z4}}Z]
  4. D. [S cdot cdot cdot cdot cdot cdot cdot cdot xrightarrow{{Z4}}Wxrightarrow{{Z1}}Xxrightarrow{{Z2}}Yxrightarrow{{Z3}}Z]
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Identify the correct statements. P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction. Q. S1 nuclease can cleave the DNA strand of a DNARNA hybrid. R. T4 polynucleotide kinase is used for labeling 3' end of DNA. S. Baculovirus (Autographa californica) can be used as an insect expression vector.

  1. A. P and Q only
  2. B. R and S only
  3. C. P and S only
  4. D. Q and R only
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CRISPR-Cas system is associated with

  1. A. Adaptive immunity in eukaryotes
  2. B. Adaptive immunity in prokaryotes
  3. C. Innate immunity in eukaryotes
  4. D. Innate immunity in prokaryotes
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Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially?

  1. A. Northern blotting
  2. B. In situ hybridization
  3. C. Southern blotting
  4. D. Western blotting
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Expressed Sequence Tag is defined as

  1. A. A partial sequence of a codon randomly selected from cDNA library
  2. B. The characteristic gene expressed in the cell
  3. C. The protein coding DNA sequence of a gene
  4. D. Uncharacterized fragment of DNA presence in the cell
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All of the following are true about DNA microarray technology except

  1. A. An electron microscope is used to gather data from the arrays
  2. B. The technology is used to assess transcription from multiple genes simultaneously
  3. C. The technology works best for organisms whose genome is completely sequenced
  4. D. The technology is derived from computer chip manufacture
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