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Biotechnology Engineering

Biochemistry In Biotechnology MCQs

Practice Biochemistry In Biotechnology MCQs for competitive exams.

Biochemistry In Biotechnology MCQs

Practice questions from this topic.

In zinc finger proteins, the amino acid residues that coordinate zinc are

  1. A. Cys and His
  2. B. Asp and Glu
  3. C. Arg and Lys
  4. D. Asp and Arg
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Which one of the following need NOT be conserved in a biochemical reaction?

  1. A. Total mass
  2. B. Total moles
  3. C. Number of atoms of each element
  4. D. Total energy
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Match the entries in Group I with the enzymes in Group II . Group I Group II P. NAD + 1. Glutathione peroxidase Q. Selenium 2. Nitrogenase R. Pyridoxal phosphate 3. Lactate dehydrogenase S. Molybdenum 4. Glycogen phosphorylase

  1. A. P-3, Q-2, R-4, S-1
  2. B. P-4, Q-1, R-3, S-2
  3. C. P-3, Q-1, R-4, S-2
  4. D. P-3, Q-4, R-2, S-1
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An enzyme catalyzes a reaction by

  1. A. Decreasing the energy of the substrate
  2. B. Decreasing the activation energy of the reaction
  3. C. Decreasing product stability
  4. D. Increasing the activation barrier of the reaction
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ATP biosynthesis takes place utilizing the H + gradient in mitochondria and chloroplasts. Identify the correct sites of H + gradient formation.

  1. A. Across the outer membrane of mitochondria and across the inner membrane of chloroplast
  2. B. Across the inner membrane of mitochondria and across the thylakoid membrane of chloroplast
  3. C. Within the matrix of mitochondria and across the inner membrane of chloroplast
  4. D. Within the matrix of mitochondria and within the stroma of chloroplast
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Which one of the following statements is CORRECT for enzyme catalyzed reactions? ( ΔG is Gibbs free energy change, K eq is equilibrium constant)

  1. A. Enzymes affect ΔG, but not K eq
  2. B. Enzymes affect K eq , but not ΔG
  3. C. Enzymes affect ΔG and K eq
  4. D. Enzymes do not affect ΔG or K eq
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In general, which one of the following statements is NOT CORRECT?

  1. A. Hydrogen bonds result from electrostatic interactions
  2. B. Hydrogen bonds contribute to the folding energy of proteins
  3. C. Hydrogen bonds are weaker than van der Waals interactions
  4. D. Hydrogen bonds are directional
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An enzymatic reaction exhibits Michaelis-Menten kinetics. For this reaction, on doubling the concentration of enzyme while maintaining [S] >> [E 0 ],

  1. A. Both K M and V max will remain the same
  2. B. K M will remain the same but V max will increase
  3. C. K M will increase but V max will remain the same
  4. D. Both K M and V max will increase
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Levinthal's paradox is related to

  1. A. Protein secretion
  2. B. Protein degradation
  3. C. Protein folding
  4. D. Protein trafficking
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Which of the following strategies are used by cells for metabolic regulation? P. Phosphorylation-dephosphorylation Q. Allostery R. Feedback inhibition

  1. A. P and Q only
  2. B. P and R only
  3. C. Q and R only
  4. D. P, Q and R
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Analysis of a hexapeptide using enzymatic cleavage reveals the following result: • Amino acid composition of the peptide is: 2R, A, V, S, Y • Trypsin digestion yields two fragments and the compositions are: (R, A, V) and (R, S, Y) • Chymotrypsin digestion yields two fragments and the compositions are: (A, R, V, Y) and (R, S) • Digestion with carboxypeptidase A yields no cleavage product. Given: Trypsin cleaves at carboxyl side of R. Chymotrypsin cleaves at carboxyl side of Y. Carboxypeptidase A cleaves at amino side of the C-terminal amino acid (except R and K) of the peptide. The correct amino acid sequence of the peptide is:

  1. A. RSYRVA
  2. B. AVRYSR
  3. C. SRYVAR
  4. D. SVRRYA
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Natural proteins are composed primarily of 20 α-amino acids. Which one of the following statements is true for any of these amino acids in a solution of pH 1.5?

  1. A. Only the amino group is ionized
  2. B. Only the carboxylic acid group is ionized
  3. C. Both amino and carboxylic acid groups are ionized
  4. D. Both amino and carboxylic add groups are neutral
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