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Biotechnology Engineering

Methods And Techniques In Applied Biology MCQs

Practice Methods And Techniques In Applied Biology MCQs for competitive exams.

Methods And Techniques In Applied Biology MCQs

Practice questions from this topic.

Which statement correctly describes sequential steps in cDNA cloning?

  1. A. Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector
  2. B. mRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase, ligation to vector
  3. C. mRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector
  4. D. Double stranded cDNA synthesis, restriction enzyme digestion, addition of linkers, ligation to vector
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Which of the following is a semi-continuous fermentation in which only a small amount of substrate is available at the beginning of the process?

  1. A. Feed-back fermentation
  2. B. Fed-batch fermentation
  3. C. Reduced Fermentation
  4. D. None of the above
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Which of the following could not possibly give rise to a restriction fragment length polymorphism (RFLP)?

  1. A. A missense mutation within the protein coding region of a gene
  2. B. A silent mutation within the protein coding region of a gene
  3. C. A single base change within the intron sequence of a gene
  4. D. An error in RNA splicing that mistakenly removes an exon during RNA processing
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Which of the following is a desirable characteristic for a cloning plasmid?

  1. A. A site at which replication can be initiated
  2. B. One or more unique restriction endonuclease sites
  3. C. One or more antibiotic-resistance or drug resistance genes
  4. D. All of the above
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Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately

  1. A. 4 : 8
  2. B. 8 : 4
  3. C. 1 : 64
  4. D. 1 : 26
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Genetically engineered male sterile crop plants may be produced by inserting

  1. A. BT toxin gene
  2. B. Barnase gene
  3. C. Lectin gene
  4. D. Chitinase gene
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ELISA is used to detect viruses where the key reagent is

  1. A. Alkaline phosphatase
  2. B. Catalase
  3. C. DNA probe
  4. D. Rnase
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Restriction endonucleases hydrolyzes polynucleotide from

  1. A. Only the 5' end
  2. B. From either terminal
  3. C. At an internal phosphodiester bond
  4. D. A phosphodiester bond within a specific sequence
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Which of the following are not valid methods of labelling duplex DNA?

  1. A. 5'-end labelling with polynucleotide kinase
  2. B. 3'-end labelling with polynucleotide kinase
  3. C. 3'-end labelling with terminal transferase
  4. D. Nick translation
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Match List-I (cloning vector) with List-II (character) and select the correct answer: List-I: Cloning vector List-II: Character a. Yeast episomal plasmid (YEp) 1. Integrates with host chromosome for replication b. Yeast integrative plasmid (YIp) 2. Carries a chromosomal origin of replication c. Yeast replicative plasmid (YRp) 3. Carries origin of replication of the 2 mm plasmid

  1. A. a-3, b-2, c-1
  2. B. a-2, b-1, c-3
  3. C. a-3, b-1, c-2
  4. D. a-2, b-3, c-1
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The genome sequencing of rice is important because

  1. A. The rice genome is very unique and contains genes not found in other plants
  2. B. The rice genome is very large compared to the DNA of other grains and so more genes will be identified
  3. C. It can identify genes associated with disease resistance, growth capacity, etc
  4. D. It has a rapid life cycle and has many identifiable mutations
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The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?

  1. A. EcoRI (G/AATTC)
  2. B. XmaIII (C/GGCGG)
  3. C. SmaI (CCC/GGG)
  4. D. SacII (CCGC/GG)
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